yflL [2019-06-25 10:32:56]

acylphosphatase

locus: BSU07640

pI: 8.67

mw: 10.18 kDa

protein length: 91 aa

gene length: 276 bp

function: unknown

product: acylphosphatase

essential: no

ec: 3.6.1.7

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 837,735 → 838,010

The protein

Catalyzed reaction/ biological activity

• An acylphosphate + H₂O = a carboxylate + phosphate (according to Swiss-Prot)

Protein family

• acylphosphatase-like domain (according to Swiss-Prot)

Structure

• [PDB|2FHM] (from ''Bacillus Subtilis'', 100% identity)
• [PDB|3BR8], [PDB|2FHM] [Pubmed|20447399]

Expression and Regulation

Operons

• genes: [gene|D7C46835B9984E2C5E375816F2BB16566202CF27|yflL]
  description: [Pubmed|22383849]

  additional information

  • 'yflL' transcription occurs in antisense orientation as compared to the surrounding '[protein|B51ADFB53CA54309D866BB089502639E9571238B|Nos]-[protein|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|YflK]' operon

Biological materials

Mutant

• MGNA-C334 (yflL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2332 NBRP B. subtilis, Japan]
• BKE07640 (Δ[gene|D7C46835B9984E2C5E375816F2BB16566202CF27|yflL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAAACCACCTTTTC, downstream forward: _UP4_GGCCATCATCGATTTTCTAT
• BKK07640 (Δ[gene|D7C46835B9984E2C5E375816F2BB16566202CF27|yflL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAAACCACCTTTTC, downstream forward: _UP4_GGCCATCATCGATTTTCTAT

References

• 20447399